Key Product Details

Species Reactivity

Viral

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 994706

View all Yellow Fever Virus NS1 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived Yellow Fever Virus NS1
Gln780-His1134
Accession # P03314

Specificity

Detects Yellow Fever Virus NS1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Viral Yellow Fever Virus NS1 Antibody

Detection of Yellow Fever Virus NS1 by Western Blot.

Western blot shows recombinant viral Yellow Fever Virus NS1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Yellow Fever Virus NS1 Monoclonal Antibody (Catalog # MAB9776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Yellow Fever Virus NS1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Viral Yellow Fever Virus NS1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant viral Yellow Fever Virus NS1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Yellow Fever Virus NS1

Yellow fever virus (YFV) non-structural protein 1 (NS1) is produced by yellow fever virus (YFV), a mosquito-borne flavivirus that is responsible for numerous epidemics of yellow fever disease with high fatality rates (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral YFV NS1 contains 352 amino acids (afa) and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

References

  1. Beasley, D.W. et al. (2015) Antiviral. Res. 115:48.
  2. Rastogi, M. et al. (2016) Virol. J. 13:131.

Alternate Names

YFV NS1, YFV-NS1, YFV_NS1

Entrez Gene IDs

1502173 (Viral)

Gene Symbol

POLY

UniProt

P03314

Additional Yellow Fever Virus NS1 Products

Product Documents for Viral Yellow Fever Virus NS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Viral Yellow Fever Virus NS1 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.