Viral wnvNS3 Protease Antibody

Name: Viral wnvNS3 Protease Antibody
Brand: R&D Systems
SKU: MAB29071
Price: 111 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB29071

Citations (2)

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Species Reactivity:	Viral
Immunogen:	E. coli-derived recombinant wnvNS3 Protease protein Ser1423-Lys1470, Gly1506-Leu1689 Accession # ABD85079
Applications:	Block/Neutralize, Immunoprecipitation, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₃ Clone # 345712
Specificity:	Detects West Nile NS3 Protease in Western blots.

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Applications for Viral wnvNS3 Protease Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE), see our available Western blot detection antibodies

Item	Value
Neutralization	Measured by its ability to neutralize Recombinant Viral wnvNS3 Protease (0.5 µg/mL, Catalog # 2907-SE) cleavage of the fluorogenic peptide substrate pERTKR-Amc (20 µM, Catalog # ES013). The Neutralization Dose (ND₅₀) is typically 15 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Viral wnvNS3 Protease Antibody

	Western Blot Detection of wnvNS3 Protease by Western Blot. Western blot shows lysates of Recombinant Viral wnvNS3 Protease. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile Tris-buffered saline, pH 7.3.
Formulation	Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein used as an immunogen consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with a G₄SG₄ linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.

Item	Value
References	Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.
Long Name	West Nile Virus NS3 Protease
Entrez Gene IDs	912267 (Viral)
Alternate Names	wnvNS3 Protease

Citations for Viral wnvNS3 Protease Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Viral wnvNS3 Protease Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Xu Z et al. (2012), West Nile virus infection causes endocytosis of a specific subset of tight junction membrane proteins. PLoS ONE, 7(5):e37886.
PMID: 22655077
Species: Human
Sample Types: Whole Cells
Applications: ICC

Xu Z et al. (2011), The capsid-binding nucleolar helicase DDX56 is important for infectivity of West Nile virus. J. Virol., 85(11):5571-80.
PMID: 21411523

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Viral wnvNS3 Protease Antibody

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Background: wnvNS3 Protease

Citations for Viral wnvNS3 Protease Antibody (2)

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