Vinculin Antibody (9I3Y8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16145
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9I3Y8 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1). DAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMM
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Vinculin Antibody (9I3Y8) (NBP3-16145) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Vinculin Antibody (9I3Y8)
Western Blot: Vinculin Antibody (9I3Y8) [NBP3-16145] -
Western Blot: Vinculin Antibody (9I3Y8) [NBP3-16145] - Western blot analysis of lysates from HeLa cells using Vinculin Rabbit mAb at 1:320000-1:1280000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (9I3Y8) [NBP3-16145] -
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (9I3Y8) [NBP3-16145] - Confocal imaging of paraffin-embedded Human colon cancer tissue using Vinculin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Vinculin Antibody (9I3Y8) [NBP3-16145] -
Analysis of lysates from PC-12 cells using Vinculin Rabbit mAb at 1:200000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.Applications for Vinculin Antibody (9I3Y8)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunocytochemistry/ Immunofluorescence
1:800 - 1:3200
Immunohistochemistry
1:800 - 1:3200
Immunohistochemistry-Paraffin
1:800 - 1:3200
Western Blot
1:50000 - 1:200000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Vinculin
Long Name
Metavinculin
Alternate Names
MVCL, VCL
Gene Symbol
VCL
Additional Vinculin Products
Product Documents for Vinculin Antibody (9I3Y8)
Product Specific Notices for Vinculin Antibody (9I3Y8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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