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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rabbit

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # BV14

Format

BSA Free

Concentration

0.5 mg/ml

Product Summary for VE-Cadherin Antibody (BV14) - BSA Free

Immunogen

The immunogen for this antibody was CD144.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34179146).

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for VE-Cadherin Antibody (BV14) - BSA Free

Western Blot: VE-Cadherin Antibody (BV14) [NBP1-43347] - Non-reduced (left) and reduced (right) bEnd.3 cell line lysates were loaded at 1x10^5 cells/lane, probed with 2 ug/mL of Anti-Mouse CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.

Applications for VE-Cadherin Antibody (BV14) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

2 ug/ml
Application Notes
Use in WB reported in scientific literature (PMID:34179146)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 7 publications using NBP1-43347 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VE-Cadherin

The BV14 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM)+ have the potential to give rise to hematopoietic cells. Cross-blocking experiments suggest that BV14 recognizes a different epitope than another mouse VE-Cadherin monoclonal antibody, BV13.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Entrez Gene IDs

12562 (Mouse)

Gene Symbol

CDH5

UniProt

Additional VE-Cadherin Products

Product Documents for VE-Cadherin Antibody (BV14) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VE-Cadherin Antibody (BV14) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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