VASP Antibody (23GB4715)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51301

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NBP3-51301-100ul
NBP3-51301-20ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB4715

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VASP Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human VASP (Uniprot: P50552)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VASP Antibody (23GB4715)

VASP Antibody (23GB4715)

Immunocytochemistry/ Immunofluorescence: VASP Antibody (23GB4715)[NBP3-51301] -

VASP antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: Vasodilator stimulated phosphoprotein shRNA knockdown (KD). Nuclei were stained blue with DAPI; Vasodilator stimulated phosphoprotein was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
VASP Antibody (23GB4715)

Immunocytochemistry/ Immunofluorescence: VASP Antibody (23GB4715)[NBP3-51301] -

Immunocytochemical staining of HT-1080 cells with VASP antibody (1:1,000). Nuclei were stained blue with DAPI; VASP was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
VASP Antibody (23GB4715)

Flow Cytometry: VASP Antibody (23GB4715)[NBP3-51301] -

Flow cytometric analysis of VASPexpression in HT-1080 cells using VASP antibody (1:2,000). Green, isotype control; red, Vasodilator stimulated phosphoprotein.
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Applications for VASP Antibody (23GB4715)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Long Name

Vasodilator-stimulated Phosphoprotein

Alternate Names

vasodilator-stimulated phosphoprotein

Gene Symbol

VASP

Additional VASP Products

Product Documents for VASP Antibody (23GB4715)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VASP Antibody (23GB4715)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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