VAMP-1 Antibody (1A3K6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16212
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1A3K6 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human VAMP-1 (P23763). MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIMLGAICAIIVVVIVIYFFT
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit VAMP-1 Antibody (1A3K6) (NBP3-16212) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for VAMP-1 Antibody (1A3K6)
![Western Blot: VAMP-1 Antibody (1A3K6) [NBP3-16212]](https://resources.bio-techne.com/images/products/VAMP-1-Antibody-1A3K6-Western-Blot-NBP3-16212-img0007.jpg "Western Blot: VAMP-1 Antibody (1A3K6) [NBP3-16212]")
Western Blot: VAMP-1 Antibody (1A3K6) [NBP3-16212]
Western Blot: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Western blot analysis of extracts of various cell lines, using VAMP-1 Rabbit mAb (NBP3-16212) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: VAMP-1 Antibody (1A3K6) [NBP3-16212] -
Immunocytochemistry/ Immunofluorescence: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunofluorescence analysis of U-2 OS cells using VAMP-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry: VAMP-1 Antibody (1A3K6) [NBP3-16212] -
Immunohistochemistry: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunohistochemistry analysis of VAMP-1 in paraffin-embedded mouse brain tissue using VAMP-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Applications for VAMP-1 Antibody (1A3K6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: VAMP-1
Long Name
Vesicle-Associated Membrane Protein 1
Alternate Names
SYB1, Synaptobrevin-1, VAMP1
Gene Symbol
VAMP1
Additional VAMP-1 Products
Product Documents for VAMP-1 Antibody (1A3K6)
Product Specific Notices for VAMP-1 Antibody (1A3K6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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