VAChT/SLC18A3 Antibody (N425/45) [FL490] - Chimeric
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52395
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
FL490
Antibody Source
Recombinant Monoclonal Chicken IgY Clone # N425/45
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Fusion protein amino acids 55-125 (first vesicle lumen domain) of rat VAChT/SLC18A3 (Uniprot#: Q62666) produced recombinantly in E. Coli
Specificity
Does not cross-react with VMAT1 or VMAT2
Clonality
Monoclonal
Host
Chicken
Isotype
IgY
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
FL490 Excitation: 491 nm, Emission: 515nm.
Each order is a unique lot. For specialized requirements, please inquire for available options.
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N425/45 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N425/45 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and conjugated.
Each order is a unique lot. For specialized requirements, please inquire for available options.
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N425/45 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N425/45 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and conjugated.
Applications for VAChT/SLC18A3 Antibody (N425/45) [FL490] - Chimeric
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
1X PBS (pH 7.4)
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: VAChT/SLC18A3
Long Name
Vesicular Acetylcholine Transporter/Solute Carrier Family 18 Member 3
Alternate Names
SLC18A3
Gene Symbol
SLC18A3
Additional VAChT/SLC18A3 Products
Product Documents for VAChT/SLC18A3 Antibody (N425/45) [FL490] - Chimeric
Product Specific Notices for VAChT/SLC18A3 Antibody (N425/45) [FL490] - Chimeric
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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