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USF1 Antibody (24GB1890)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50653

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NBP3-50653-100ul
NBP3-50653-20ul

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB1890

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human USF1 (Uniprot: P22415)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USF1 Antibody (24GB1890)

USF1 Antibody (24GB1890)

Immunocytochemistry/ Immunofluorescence: USF1 Antibody (24GB1890)[NBP3-50653] -

Immunocytochemical staining of HepG2 cells with USF1 antibody (1:1,000). Nuclei were stained blue with DAPI; USF1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
USF1 Antibody (24GB1890)

Flow Cytometry: USF1 Antibody (24GB1890)[NBP3-50653] -

Flow cytometric analysis of USF1expression in HepG2 cells using USF1 antibody (1:2,000). Green, isotype control; red, Upstream transcription factor 1.
USF1 Antibody (24GB1890)

Western Blot: USF1 Antibody (24GB1890)[NBP3-50653] -

Western blotting analysis using USF1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for USF1 Antibody (24GB1890)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USF1

USF1 encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

BHLHB11, bHLHb11FCHL1, Class B basic helix-loop-helix protein 11, FCHL, HYPLIP1, Major late transcription factor 1, MLTF, MLTFI, UEF, upstream stimulatory factor 1, upstream transcription factor 1, USF

Gene Symbol

USF1

Additional USF1 Products

Product Documents for USF1 Antibody (24GB1890)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for USF1 Antibody (24GB1890)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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