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TRACP/PAP/ACP5 Antibody (7E6A11) - BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-37337

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NBP2-37337
NBP2-37337-0.025ml

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7E6A11

Format

BSA Free

Concentration

1 mg/ml

Product Summary for TRACP/PAP/ACP5 Antibody (7E6A11) - BSA Free

Immunogen

Purified recombinant fragment of human TRACP/PAP/ACP5 (AA: 221-325) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRACP/PAP/ACP5 Antibody (7E6A11) - BSA Free

Western Blot: TRACP/PAP/ACP5 Antibody (7E6A11) [NBP2-37337] - Western blot analysis using ACP5 mouse mAb against A431 (1), T47D (2), HepG2 (3), MOLT4 (4), Jurkat (5) and Hela (6) cell lysate.
Immunohistochemistry: TRACP/PAP/ACP5 Antibody (7E6A11) [NBP2-37337] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ACP5 mouse mAb with DAB staining.
Western Blot: TRACP/PAP/ACP5 Antibody (7E6A11) [NBP2-37337] - Western blot analysis using ACP5 mAb against human ACP5 recombinant protein. (Expected MW is 37.3 kDa)

Applications for TRACP/PAP/ACP5 Antibody (7E6A11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRACP/PAP/ACP5

TRACP is an iron-containing, 35 kDa glycoprotein that hydrolyses phosphomonoesters. It is produced as a monomer by osteoclasts and is cleaved by cathepsins to create a disulfide-linked dimer that acts on bone matrix phosphoserine-containing proteins in lysosomal vesicles. Following phosphate cleavage, TRACP is released from the cell where it serves as a marker of bone resorption.

Long Name

Tartrate Resistant Acid Phosphatase 5

Alternate Names

ACP5, TRACP-5b, TTRRAP

Gene Symbol

ACP5

Additional TRACP/PAP/ACP5 Products

Product Documents for TRACP/PAP/ACP5 Antibody (7E6A11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRACP/PAP/ACP5 Antibody (7E6A11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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