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TGF-beta 2/1.2 Antibody

Bio-Techne includes R&D Systems | Catalog # AF-302-NA

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AF-302-NA
AF-302-SP

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Canine

Applications

Immunohistochemistry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for TGF-beta 2/1.2 Antibody

Immunogen

Porcine platelet-derived TGF-beta 2

Specificity

Detects TGF‑ beta2/1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 2% cross‑reactivity with TGF-beta 1, TGF-beta 3, and TGF-beta 5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TGF-beta 2/1.2 Antibody

TGF-beta 2 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 2/1.2 Antibody.

TGF-beta 2 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 2/1.2 Antibody.

Porcine TGF-beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-TGF-beta 2/1.2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-302-NA). The ND50 is typically 0.01-0.03 µg/mL.

Applications for TGF-beta 2/1.2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human TGF-beta 2 (Catalog # 302-B2)
Recombinant Human TGF-beta 1.2 (Catalog # 304-B3)

Neutralization

Measured by its ability to neutralize TGF-beta 2 inhibition of IL-4-dependent proliferation in the HT-2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 1 ng/mL Porcine TGF-beta 2 and 7.5 ng/mL Recombinant Mouse IL-4.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using AF-302-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 2/1.2

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

Long Name

Transforming Growth Factor beta 1 and 1.2

Entrez Gene IDs

7042 (Human)

Gene Symbol

TGFB2

Additional TGF-beta 2/1.2 Products

Product Documents for TGF-beta 2/1.2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for TGF-beta 2/1.2 Antibody

For research use only

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