t-Plasminogen Activator/tPA Antibody (10W4L1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16356
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10W4L1 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 463-562 of human tPA/t-Plasminogen Activator/tPA (P00750). LKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit t-Plasminogen Activator/tPA Antibody (10W4L1) (NBP3-16356) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for t-Plasminogen Activator/tPA Antibody (10W4L1)
![Western Blot: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356]](https://resources.bio-techne.com/images/products/t-Plasminogen-Activator-tPA-Antibody-10W4L1-Western-Blot-NBP3-16356-img0002.jpg "Western Blot: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356]")
Western Blot: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356]
Western Blot: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356] - Western blot analysis of extracts of various cell lines, using tPA/t-Plasminogen Activator/tPA Rabbit mAb (NBP3-16356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356] -
Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356] - NIH/3T3 cells using tPA/PLAT Rabbit mAb (at dilution of 1:100) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Applications for t-Plasminogen Activator/tPA Antibody (10W4L1)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunocytochemistry/ Immunofluorescence
1:100 - 1:400
Western Blot
1:1000 - 1:6000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: t-Plasminogen Activator/tPA
Long Name
Tissue Plasminogen Activator
Alternate Names
Alteplase, PLAT, Reteplase, T-PA, tPlasminogen Activator
Gene Symbol
PLAT
Additional t-Plasminogen Activator/tPA Products
Product Documents for t-Plasminogen Activator/tPA Antibody (10W4L1)
Product Specific Notices for t-Plasminogen Activator/tPA Antibody (10W4L1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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