Staining of U-251 cells, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody (CL0257) [NBP3-52445]

Staining of human kidney shows strong apical membrane positivity in cells in proximal tubules.

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP3-52441]

Staining of human lymph node shows moderate to strong cytoplasmic positivity in lymphoid cells.

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Applications for Syntaxin 7 Antibody (CL0257)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syntaxin 7

Syntaxin 7 is involved in the ordered fusion of endosomes and lysosomes with the phagosome, where phagocytic cells kill and degrade internalized foreign particles. Syntaxin 7 is found in late endosomes and lysosomes; whereas Syntaxin 12 is localized to the recycling endosome compartment, both are recruited to the phagosome. However, STX12 is acquired earlier before rapidly recycling off the phagosome, whereas STX7 is recruited later and continues to accumulate throughout the phagosome maturation process.

Alternate Names

STX7

Gene Symbol

STX7

Additional Syntaxin 7 Products

Product Documents for Syntaxin 7 Antibody (CL0257)

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Syntaxin 7 Antibody (CL0257)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Syntaxin 7 Antibody (CL0257)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52445

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all Syntaxin 7 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for Syntaxin 7 Antibody (CL0257)

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody (CL0257) [NBP3-52445]

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody (CL0257) [NBP3-52445]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP3-52441]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0143) [NBP3-52442]

Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP3-52444]

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP3-52448]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP3-52446]

Applications for Syntaxin 7 Antibody (CL0257)

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Reconstitution

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Syntaxin 7

Alternate Names

Gene Symbol

Additional Syntaxin 7 Products

Product Documents for Syntaxin 7 Antibody (CL0257)

Product Datasheets

COA

Certificate of Analysis

Product Specific Notices for Syntaxin 7 Antibody (CL0257)