SSEA-4 Antibody (MC-813-70) [Janelia Fluor® 669]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26644JF669
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Canine, Chicken, Primate, Rabbit
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence
Label
Janelia Fluor 669
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # MC-813-70
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human embryonal carcinoma cell line 2102Ep
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Applications for SSEA-4 Antibody (MC-813-70) [Janelia Fluor® 669]
Application
Recommended Usage
Flow (Cell Surface)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: SSEA-4
SSEAs were originally identified by monoclonal antibodies recognizing defined carbohydrate epitopes associated with lacto- and globo-series glycolipids. They are often used as markers for stem cell differentiation. CD15/Lewis X/SSEA-1 is expressed on murine embryonal carcinoma (EC) cells, embryonic stem (ES) cells, and primordial germ cells. SSEA-3 and SSEA-4 are synthesized during oogenesis and is present on oocyte, zygote, and early cleavage-stage embryo membranes. Mouse CD15/Lewis X/SSEA-1 expression decreases with differentiation as SSEA-3 and SSEA-4 expression increase. In contrast, human EC and ES cells express SSEA-3 and SSEA-4, and differentiation is accompanied by an upregulation of CD15/Lewis X/SSEA-1 and down-regulation of SSEA-3 and SSEA-4.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4
Additional SSEA-4 Products
Product Documents for SSEA-4 Antibody (MC-813-70) [Janelia Fluor® 669]
Product Specific Notices for SSEA-4 Antibody (MC-813-70) [Janelia Fluor® 669]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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