SSEA-4 Antibody (MC-813-70) [Alexa Fluor™ Plus 488]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26644AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Canine, Chicken, Primate, Rabbit
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence
Label
Alexa Fluor Plus 488
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # MC-813-70
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human embryonal carcinoma cell line 2102Ep
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Applications for SSEA-4 Antibody (MC-813-70) [Alexa Fluor™ Plus 488]
Application
Recommended Usage
Flow (Cell Surface)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: SSEA-4
SSEAs were originally identified by monoclonal antibodies recognizing defined carbohydrate epitopes associated with lacto- and globo-series glycolipids. They are often used as markers for stem cell differentiation. CD15/Lewis X/SSEA-1 is expressed on murine embryonal carcinoma (EC) cells, embryonic stem (ES) cells, and primordial germ cells. SSEA-3 and SSEA-4 are synthesized during oogenesis and is present on oocyte, zygote, and early cleavage-stage embryo membranes. Mouse CD15/Lewis X/SSEA-1 expression decreases with differentiation as SSEA-3 and SSEA-4 expression increase. In contrast, human EC and ES cells express SSEA-3 and SSEA-4, and differentiation is accompanied by an upregulation of CD15/Lewis X/SSEA-1 and down-regulation of SSEA-3 and SSEA-4.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4
Additional SSEA-4 Products
Product Documents for SSEA-4 Antibody (MC-813-70) [Alexa Fluor™ Plus 488]
Product Specific Notices for SSEA-4 Antibody (MC-813-70) [Alexa Fluor™ Plus 488]
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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