SPATA5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38302
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SLELSLQLSQLDLEDTQIPTSRSTPYKPIDDRITNKASDVLLDVTQSPGDGSGLMLEEVTGLKCNFESAREGNEQLT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SPATA5 Antibody - BSA Free
Western Blot: SPATA5 Antibody [NBP2-38302]
Western Blot: SPATA5 Antibody [NBP2-38302] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissueImmunohistochemistry-Paraffin: SPATA5 Antibody [NBP2-38302]
Immunohistochemistry-Paraffin: SPATA5 Antibody [NBP2-38302] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.Western Blot: SPATA5 Antibody - BSA Free [NBP2-38302] -
Genetic reconstitution of viral infection by expression of SBDS and SPATA5. (A) Infectivity of Huh7.5 cells treated with indicated CRISPR gRNAs and complemented with lentiviral expression of firefly luciferase (Fluc) or guide-resistant host factors infected with MOI 1 YFV-Venus for 24 h and analyzed by flow cytometry. (B) Lysates from Huh7.5 cells untreated or treated with indicated CRISPR gRNAs and complemented with lentiviral expression of Fluc or guide-resistant host factors were analyzed by Western blotting using anti-SBDS or anti-SPATA5 antibodies. Blot membranes were also probed with anti-actin antibodies (indicated) (n = 3 biological replicates). Statistical significance was determined by two-way ANOVA. KO, knockout; NonTarg or NT, nontargeting CRISPR guides; WT, wild type; ****, P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36809113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for SPATA5 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SPATA5
Alternate Names
AFG2ATPase family gene 2 homolog, ATPase family protein 2 homolog, SPAFspermatogenesis associated factor SPAF, spermatogenesis associated 5, Spermatogenesis-associated factor protein, spermatogenesis-associated protein 5
Gene Symbol
AFG2A
UniProt
Additional SPATA5 Products
Product Documents for SPATA5 Antibody - BSA Free
Product Specific Notices for SPATA5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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