Sonic Hedgehog/Shh Antibody (24GB1925)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51594

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NBP3-51594-100ul
NBP3-51594-20ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB1925

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sonic Hedgehog/Shh Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Sonic Hedgehog/Shh (Uniprot: Q15465)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sonic Hedgehog/Shh Antibody (24GB1925)

Sonic Hedgehog/Shh Antibody (24GB1925)

Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (24GB1925)[NBP3-51594] -

Immunocytochemical staining of HepG2 cells with Sonic Hedgehog/Shh antibody (1:1,000). Nuclei were stained blue with DAPI; Sonic hedgehog signaling molecule was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Sonic Hedgehog/Shh Antibody (24GB1925)

Flow Cytometry: Sonic Hedgehog/Shh Antibody (24GB1925)[NBP3-51594] -

Flow cytometric analysis of Sonic Hedgehog/Shhexpression in HepG2 cells using Sonic Hedgehog/Shh antibody (1:2,000). Green, isotype control; red, Sonic hedgehog signaling molecule.
Sonic Hedgehog/Shh Antibody (24GB1925)

Western Blot: Sonic Hedgehog/Shh Antibody (24GB1925)[NBP3-51594] -

Western blotting analysis using Sonic Hedgehog/Shh antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for Sonic Hedgehog/Shh Antibody (24GB1925)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sonic Hedgehog/Shh

Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal (1). Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene (2). Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo (3). A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma (4).

Alternate Names

HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS

Gene Symbol

SHH

Additional Sonic Hedgehog/Shh Products

Product Documents for Sonic Hedgehog/Shh Antibody (24GB1925)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Sonic Hedgehog/Shh Antibody (24GB1925)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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