Smad4 Antibody (CL9774) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05507
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]](https://resources.bio-techne.com/images/products/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0002.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]")
![Western Blot: Smad4 Antibody (CL9774) [NBP3-05507]](https://resources.bio-techne.com/images/products/Smad4-Antibody-CL9774-Western-Blot-NBP3-05507-img0006.jpg "Western Blot: Smad4 Antibody (CL9774) [NBP3-05507]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL9774
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q13485, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Smad4 Antibody (CL9774) - BSA Free
Western Blot: Smad4 Antibody (CL9774) [NBP3-05507]
Western Blot: Smad4 Antibody (CL9774) [NBP3-05507] - Analysis in human cell line SCLC-21H.![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]](https://resources.bio-techne.com/images/products/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0005.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]")
Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]
Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]](https://resources.bio-techne.com/images/products/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0001.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]")
Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]
Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human placenta shows moderate nuclear and cytoplasmic positivity in trophoblastic cells.![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]](https://resources.bio-techne.com/images/products/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0003.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]")
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]](https://resources.bio-techne.com/images/products/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0004.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507]")
Applications for Smad4 Antibody (CL9774) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Smad4
Long Name
Mothers Against DPP Homolog 4
Alternate Names
DPC4, MADH4
Gene Symbol
SMAD4
Additional Smad4 Products
Product Documents for Smad4 Antibody (CL9774) - BSA Free
Product Specific Notices for Smad4 Antibody (CL9774) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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