Smad2 Antibody (CL8472) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44228

Novus Biologicals, part of Bio-Techne
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NBP3-44228
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BSA Free
NBP2-88916
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL8472

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Smad2 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q15796, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Novus Biologicals Mouse Smad2 Antibody (CL8472) - Azide and BSA Free (NBP2-88916) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Smad2 Antibody (CL8472) - Azide and BSA Free

Smad2 Antibody (CL8472) - Azide and BSA Free Immunohistochemistry-Paraffin: Smad2 Antibody [NBP3-44228]

Immunohistochemistry-Paraffin: Smad2 Antibody [NBP3-44228]

Analysis in human fallopian tube and pancreas tissues using NBP3-44228 antibody. Corresponding Smad2 RNA-seq data are presented for the same tissues.
Smad2 Antibody (CL8472) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Smad2 Antibody [NBP3-44228]

Immunocytochemistry/ Immunofluorescence: Smad2 Antibody [NBP3-44228]

Staining of HeLa cells using the Anti-Smad2 monoclonal antibody) .
Smad2 Antibody (CL8472) - Azide and BSA Free Immunohistochemistry-Paraffin: Smad2 Antibody [NBP3-44228]

Immunohistochemistry-Paraffin: Smad2 Antibody [NBP3-44228]

Staining of human breast cancer shows moderate cytoplasmic positivity.
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Applications for Smad2 Antibody (CL8472) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad2

Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).

Long Name

Mothers Against DPP Homolog 2

Alternate Names

JV18-1, MADH2, MADR2, Smox

Gene Symbol

SMAD2

Additional Smad2 Products

Product Documents for Smad2 Antibody (CL8472) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Smad2 Antibody (CL8472) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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