SMAD1/5/9 [p Ser467] Antibody (23GB4155)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50322

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NBP3-50322-100ul
NBP3-50322-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB4155

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SMAD1/5/9 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-SMAD1/5/9(S463/S465/S467) (Uniprot: Q15797/Q99717/O15198)

Modification

p Ser467

Specificity

This antibody detects endogenous levels of SMAD1/5/9 only when phosphorylated at S463 or S465 or S467

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMAD1/5/9 [p Ser467] Antibody (23GB4155)

SMAD1/5/9 [p Ser467] Antibody (23GB4155)

Immunocytochemistry/ Immunofluorescence: SMAD1/5/9 [p Ser467] Antibody (23GB4155)[NBP3-50322] -

Immunocytochemical staining of HAP-1 cells with SMAD1/5/9 (1:1,000). Nuclei were stained blue with DAPI; Phospho-Smad1/5/9 (S463/S465/S467) was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
SMAD1/5/9 [p Ser467] Antibody (23GB4155)

Flow Cytometry: SMAD1/5/9 [p Ser467] Antibody (23GB4155)[NBP3-50322] -

Flow cytometric analysis of SMAD1/5/9expression in HAP-1 cells using SMAD1/5/9 antibody (1:2,000). Green, isotype control; red, Phospho-Smad1/5/9 (S463/S465/S467).
SMAD1/5/9 [p Ser467] Antibody (23GB4155)

Western Blot: SMAD1/5/9 [p Ser467] Antibody (23GB4155)[NBP3-50322] -

Western blotting analysis using SMAD1/5/9 (S463/S465/S467) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for SMAD1/5/9 [p Ser467] Antibody (23GB4155)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMAD1/5/9

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Alternate Names

BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1

Gene Symbol

SMAD1

Additional SMAD1/5/9 Products

Product Documents for SMAD1/5/9 [p Ser467] Antibody (23GB4155)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SMAD1/5/9 [p Ser467] Antibody (23GB4155)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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