Siglec-3/CD33 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32709

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NBP2-32709
NBP2-32709-25ul
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Conjugate
Catalog #
Biotin
NBP3-71261
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Siglec-3/CD33 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KTHRRKAARTAVGRNDTHPTTGSASPKHQKKSKLHGPTETSSCSGAAPTVEMDEELHYASLNFHGMNPSKDTSTEYSEVRTQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Siglec-3/CD33 Antibody - BSA Free

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709]

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709]

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709]

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709]

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human lung shows moderate positivity in macrophages.
Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709]

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709]

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human lymph node shows moderate positivity in lymphoid cells.
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Applications for Siglec-3/CD33 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-3/CD33

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Gene Symbol

CD33

Additional Siglec-3/CD33 Products

Product Documents for Siglec-3/CD33 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Siglec-3/CD33 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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