Siglec-3/CD33 Antibody (WM53) - Low Endotoxin
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09095
Clone WM53 was used by HLDA to establish CD designation.
![Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095]](https://resources.bio-techne.com/images/products/Siglec-3-CD33-Antibody-WM53-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09095-img0002.jpg "Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # WM53
Format
Low Endotoxin
Concentration
1 mg/ml
Product Specifications
Immunogen
Human AML cells
Specificity
The mouse monoclonal antibody WM53 reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Scientific Data Images for Siglec-3/CD33 Antibody (WM53) - Low Endotoxin
Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095]
Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Mass cytometry (surface staining) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.![Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095]](https://resources.bio-techne.com/images/products/Siglec-3-CD33-Antibody-WM53-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09095-img0001.jpg "Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095]")
Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095]
Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Analysis (surface staining) of human peripheral blood with anti-CD33 (WM53) purified antibody (low endotoxin), GAM-APC.Applications for Siglec-3/CD33 Antibody (WM53) - Low Endotoxin
Application
Recommended Usage
Flow Cytometry
1-4 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
Low Endotoxin
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Siglec-3/CD33
Long Name
Sialic Acid Binding Ig-like Lectin 3
Alternate Names
CD33, gp67, Siglec3
Gene Symbol
CD33
Additional Siglec-3/CD33 Products
Product Documents for Siglec-3/CD33 Antibody (WM53) - Low Endotoxin
Product Specific Notices for Siglec-3/CD33 Antibody (WM53) - Low Endotoxin
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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