Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08990

Recombinant Monoclonal Antibody
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NBP3-08990-0.025mg
NBP3-08990-0.1mg
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2a Kappa Clone # 3D6.112 (recombinant version)

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Siglec-1/CD169 Antibodies »

Product Specifications

Immunogen

Spleen cells from AO rats immunised with thioglycollate-elicted murine peritoneal macrophages (TPM) that had been cutured in mouse serum to induce sheep erythrocyte receptor (SER).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images

Flow Cytometry: Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) - Azide and BSA Free [NBP3-08990]

Flow Cytometry: Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) - Azide and BSA Free [NBP3-08990]

Flow Cytometry: Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) [NBP3-08990] - Murine bone marrow-derived macrophages (BMDMs) were stained with anti-Siglec-1/CD169 antibody NBP3-08990 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor(R) 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a Siglec-1/CD169-positive population is apparent.

Applications

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This constant region of this antibody has been modified to bind to Protein A, allowing highly-specific purification of this antibody by Protein A-affinity column.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-1/CD169

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Gene Symbol

SIGLEC1

Additional Siglec-1/CD169 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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