SHP-1 Antibody (PTPN6/7543) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26999
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PTPN6/7543
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa395-595) of human SHP-1 protein (exact sequence is proprietary)
Localization
Cytoplasm, Nucleus, In neurons, translocates into the nucleus after treatment with angiotensin II,
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for SHP-1 Antibody (PTPN6/7543) - Azide and BSA Free
Immunohistochemistry-Paraffin: SHP-1 Antibody (PTPN6/7543) - Azide and BSA Free [NBP3-26999] -
Formalin-fixed, paraffin-embedded human spleen stained with SHP-1 Antibody (PTPN6/7543) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: SHP-1 Antibody (PTPN6/7543) - Azide and BSA Free [NBP3-26999] -
Formalin-fixed, paraffin-embedded human spleen stained with SHP-1 Antibody (PTPN6/7543) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.Applications for SHP-1 Antibody (PTPN6/7543) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml for 30 minutes at RT
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: SHP-1
The SH2 domain-containing phosphatases, SHP-1 and SHP-2, are well-studied cytosolic tyrosine phosphatases that share many structural and regulatory features. They both have two tandem SH2 domains at the N-terminus, followed by a catalytic domain, and an inhibitory C-terminus. Despite their structural similarity, the enzymes appear to have different physiological roles and exhibit different expression patterns. SHP-1 is highly expressed in hematopoietic tissues, and in general has a negative impact on lymphocyte signaling. In contrast, SHP-2 is widely expressed, and in general promotes signaling pathways that lead to differentiation, cell growth, or migration.
Long Name
Src Homology Region 2 Domain Phosphatase 1
Alternate Names
HCPH, PTP1C, PTPN6, SHP1
Gene Symbol
PTPN6
Additional SHP-1 Products
Product Documents for SHP-1 Antibody (PTPN6/7543) - Azide and BSA Free
Product Specific Notices for SHP-1 Antibody (PTPN6/7543) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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