SHP-1 Antibody (M160)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23394
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M160
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone (M160) was generated from a recombinant protein containing amino acids in the C-terminal region of human SHP-1. This sequence is highly conserved in rat and mouse SHP-1. [UniProt# P29350]
Specificity
The antibody detects a 68 kDa protein in human A431 and Jurkat cells, and does not cross-react with SHP2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for SHP-1 Antibody (M160)
Western Blot-SHP-1 Antibody (M160)-NBP3-23394-
Western Blot-SHP-1 Antibody (M160)-NBP3-23394-Analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti- SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Ser-591) antibody (lanes 3-6). The SHP1 (Ser-591) antibody was used in the presence of phospho-SHP1 (Ser-591) peptide (lane 5) or a non-specific phospho- serine peptide (lane 6).Applications for SHP-1 Antibody (M160)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunoprecipitation
1:100
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: SHP-1
The SH2 domain-containing phosphatases, SHP-1 and SHP-2, are well-studied cytosolic tyrosine phosphatases that share many structural and regulatory features. They both have two tandem SH2 domains at the N-terminus, followed by a catalytic domain, and an inhibitory C-terminus. Despite their structural similarity, the enzymes appear to have different physiological roles and exhibit different expression patterns. SHP-1 is highly expressed in hematopoietic tissues, and in general has a negative impact on lymphocyte signaling. In contrast, SHP-2 is widely expressed, and in general promotes signaling pathways that lead to differentiation, cell growth, or migration.
Long Name
Src Homology Region 2 Domain Phosphatase 1
Alternate Names
HCPH, PTP1C, PTPN6, SHP1
Gene Symbol
PTPN6
Additional SHP-1 Products
Product Documents for SHP-1 Antibody (M160)
Product Specific Notices for SHP-1 Antibody (M160)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...