Western blot shows recombinant SARS-CoV-2 envelope. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 Envelope Monoclonal Antibody (Catalog # MAB10894) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Envelope at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for SARS-CoV-2 Envelope Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: Recombinant SARS-CoV-2 envelope protein

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Envelope

The SARS-CoV-2 Envelope protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). The envelope protein is the smallest of the four structural proteins, which also includes the membrane protein, spike protein, and nucleocapsid protein (1, 2). The envelope protein is synthesized as a 75 amino acid protein with a theoretical molecular weight of approximately 8.4 kDa (2, 3). Furthermore, the envelope protein of SARS-CoV-2 has 94.7% sequence identity and 97.4% sequence similarity to the envelope protein of SARS-CoV (2). Structurally, the envelope protein is a membrane protein with a N-terminal domain, an alpha-helical transmembrane domain, and a hydrophilic C-terminal domain (1,4). The envelope protein has multiple functions in viral replication including viral assembly, release, and pathogenesis (2,4). Additionally, the SARS-CoV-2 envelope protein has ion channel activity and functions as a viroporin with a role in virion trafficking (2, 4). Coronaviruses lacking the envelope protein are shown to have reduced viral titer and slowed or defective maturation, indicative of a role in virus production and growth (4).

References

Malik, Y.A. (2020) Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.
Yoshimoto F.K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4.
Uniprot (P0DTC4).
J. Alsaadi, E.A. & Jones, I. M. (2019). Membrane binding proteins of coronaviruses. Future Virology. https://doi.org/10.2217/fvl-2018-0144.

Long Name

Envelope Protein

Alternate Names

E Protein

Entrez Gene IDs

43740570 (SARS-CoV-2)

Gene Symbol

UniProt

YP_009724392

Additional Envelope Products

Product Documents for SARS-CoV-2 Envelope Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for SARS-CoV-2 Envelope Antibody

For research use only

Citations
Reviews

SARS-CoV-2 Envelope Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10894

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Envelope Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for SARS-CoV-2 Envelope Antibody

Detection of SARS-CoV-2 Envelope by Western Blot.