SAP155 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55255

Novus Biologicals, part of Bio-Techne
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NB100-55255
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Simple Western, Immunoprecipitation, Knockdown Validated

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all SAP155 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human splicing factor 3b, subunit 1, 155kDa using the numbering given in entry NP_036565.2 (GeneID 23451).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SAP155 Antibody (NB100-55255) is a polyclonal antibody validated for use in WB, Simple Western and IP. Anti-SAP155 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SAP155 Antibody

Western Blot: SAP155 Antibody [NB100-55255]

Western Blot: SAP155 Antibody [NB100-55255]

Western Blot: SAP155 Antibody [NB100-55255] - Detection of Human SF3b155/SAP155 on HeLa whole cell lysate using NB100-55255. SF3b155/SAP155 was also immunoprecipitated by rabbit anti-SF3b155/SAP155 antibody NB100-55256.
Knockdown Validated: SAP155 Antibody [NB100-55255]

Western Blot: SAP155 Antibody [NB100-55255]

SAP155-Antibody-Knockdown-Validated-NB100-55255-img0003.jpg

Applications for SAP155 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in SW reported in secitific publication PMID: 32427851
See Simple Western Antibody Database for Simple Western validation: separated by Size

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SAP155

SAP155 is a subunit of the splicing factor SF3B required for SF3B155 is a subunit of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) that functions in pre-mRNA splicing. SF3B155 together with splicing factor 3a binds pre-mRNA upstream of the branchpoint sequence to anchor U2 snRNP to the pre-mRNA. SF3B155 is also a component of the U12-type splicesome that is involved in the splicing of a rare class of nuclear pre-mRNA introns.'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of the SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. It may also be involved in the assembly of the 'E' complex. SAP155 also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Alternate Names

Hsh155, pre-mRNA processing 10, pre-mRNA splicing factor SF3b, 155 kDa subunit, Pre-mRNA-splicing factor SF3b 155 kDa subunit, Prp10, PRPF10, SAP 155, SAP155splicing factor 3b, subunit 1, 155kD, SF3B155, SF3b155PRP10, spliceosome associated protein 155, Spliceosome-associated protein 155, splicing factor 3B subunit 1, splicing factor 3b, subunit 1, 155kDa

Entrez Gene IDs

23451 (Human)

Gene Symbol

SF3B1

UniProt

O75533 (Human)

Additional SAP155 Products

Product Documents for SAP155 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SAP155 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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