ROCK2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20199
![Western Blot: ROCK2 Antibody [NBP2-20199]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-Western-Blot-NBP2-20199-img0001.jpg "Western Blot: ROCK2 Antibody [NBP2-20199]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.
Localization
Cytoplasm (By similarity); Cell membrane; Peripheral membrane protein (By similarity)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit ROCK2 Antibody - BSA Free (NBP2-20199) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ROCK2 Antibody - BSA Free
![Western Blot: ROCK2 Antibody [NBP2-20199]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-Western-Blot-NBP2-20199-img0008.jpg "Western Blot: ROCK2 Antibody [NBP2-20199]")
Western Blot: ROCK2 Antibody [NBP2-20199]
Western Blot: ROCK2 Antibody [NBP2-20199] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [N3C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody [NBP2-20199]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20199-img0005.jpg "Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody [NBP2-20199]")
Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody [NBP2-20199]
Immunocytochemistry/Immunofluorescence: ROCK2 Antibody [NBP2-20199] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.![Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-20199-img0004.jpg "Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199]")
Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199]
Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199] - Human esophagous cancer, using ROCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: ROCK2 Antibody [NBP2-20199]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-Western-Blot-NBP2-20199-img0002.jpg "Western Blot: ROCK2 Antibody [NBP2-20199]")
![Western Blot: ROCK2 Antibody [NBP2-20199]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-Western-Blot-NBP2-20199-img0003.jpg "Western Blot: ROCK2 Antibody [NBP2-20199]")
![Knockdown Validated: ROCK2 Antibody [NBP2-20199]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-Western-Blot-NBP2-20199-img0006.jpg "Western Blot: ROCK2 Antibody [NBP2-20199]")
Applications for ROCK2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ROCK2
Long Name
Rho-associated, Coiled-coil Containing Protein Kinase 2
Alternate Names
ROCK-II, ROKalpha
Gene Symbol
ROCK2
UniProt
Additional ROCK2 Products
Product Documents for ROCK2 Antibody - BSA Free
Product Specific Notices for ROCK2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.