ROCK2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-593
![Western Blot: ROCK2 Antibody [NB100-593]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-Western-Blot-NB100-593-img0002.jpg "Western Blot: ROCK2 Antibody [NB100-593]")
![Western Blot: ROCK2 Antibody [NB100-593]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-Western-Blot-NB100-593-img0006.jpg "Western Blot: ROCK2 Antibody [NB100-593]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 1320 and the C-terminus (residues 1388) of human ROCK2 using the number given in entry NP_004841.1 (GeneID 9475)
Specificity
NB100-593 is specific for human Rho Kinase alpha protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ROCK2 Antibody - BSA Free (NB100-593) is a polyclonal antibody validated for use in WB and IP. Anti-ROCK2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ROCK2 Antibody - BSA Free
Western Blot: ROCK2 Antibody [NB100-593]
Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for ROCK2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:10000-1:25000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000 Immunoprecipitation: 2 to 5 ug/mg of lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ROCK2
Long Name
Rho-associated, Coiled-coil Containing Protein Kinase 2
Alternate Names
ROCK-II, ROKalpha
Entrez Gene IDs
9475 (Human)
Gene Symbol
ROCK2
UniProt
Additional ROCK2 Products
Product Documents for ROCK2 Antibody - BSA Free
Product Specific Notices for ROCK2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...