ROCK2 Antibody (GT11712) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13535

Novus Biologicals, part of Bio-Techne
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NBP3-13535
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT11712

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ROCK2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (81%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ROCK2 Antibody (GT11712) - Azide and BSA Free

Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535]

Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535]

Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: ROCK2 Antibody (GT11712) [NBP3-13535]

Immunohistochemistry-Paraffin: ROCK2 Antibody (GT11712) [NBP3-13535]

Immunohistochemistry-Paraffin: ROCK2 Antibody (GT11712) [NBP3-13535] - ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535]

Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535]

Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:500.
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Applications for ROCK2 Antibody (GT11712) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROCK2

ROCK2 is encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Long Name

Rho-associated, Coiled-coil Containing Protein Kinase 2

Alternate Names

ROCK-II, ROKalpha

Gene Symbol

ROCK2

Additional ROCK2 Products

Product Documents for ROCK2 Antibody (GT11712) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ROCK2 Antibody (GT11712) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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