ROCK2 Antibody (8G3U2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16123
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8G3U2 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1289-1388 of human ROCK2 (O75116). PALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit ROCK2 Antibody (8G3U2) (NBP3-16123) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ROCK2 Antibody (8G3U2)
![Western Blot: ROCK2 Antibody (8G3U2) [NBP3-16123]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-8G3U2-Western-Blot-NBP3-16123-img0002.jpg "Western Blot: ROCK2 Antibody (8G3U2) [NBP3-16123]")
Western Blot: ROCK2 Antibody (8G3U2) [NBP3-16123]
Western Blot: ROCK2 Antibody (8G3U2) [NBP3-16123] - Western blot analysis of extracts of various cell lines, using ROCK2 Rabbit mAb (NBP3-16123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: ROCK2 Antibody (8G3U2) [NBP3-16123] -
Immunohistochemistry-Paraffin: ROCK2 Antibody (8G3U2) [NBP3-16123] - Analysis of paraffin-embedded Rat colon tissue using ROCK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry-Paraffin: ROCK2 Antibody (8G3U2) [NBP3-16123] -
Immunohistochemistry-Paraffin: ROCK2 Antibody (8G3U2) [NBP3-16123] - Analysis of paraffin-embedded Mouse intestin tissue using ROCK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.Applications for ROCK2 Antibody (8G3U2)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunohistochemistry
1:200 - 1:800
Immunohistochemistry-Paraffin
1:200 - 1:800
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ROCK2
Long Name
Rho-associated, Coiled-coil Containing Protein Kinase 2
Alternate Names
ROCK-II, ROKalpha
Gene Symbol
ROCK2
Additional ROCK2 Products
Product Documents for ROCK2 Antibody (8G3U2)
Product Specific Notices for ROCK2 Antibody (8G3U2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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