ROCK2 Antibody (23GB2030)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50978
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB2030
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human ROCK2 (Uniprot: O75116)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ROCK2 Antibody (23GB2030)
Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (23GB2030)[NBP3-50978] -
ROCK2 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: ROCK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ROCK2 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (23GB2030)[NBP3-50978] -
Immunocytochemical staining of HepG2 cells with ROCK2 antibody (1:1,000). Nuclei were stained blue with DAPI; ROCK2 cochaperone 3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: ROCK2 Antibody (23GB2030)[NBP3-50978] -
Validation of ROCK2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ROCK2 antibody (1:2,000) and analyzed using BD flow cytometer.
Applications for ROCK2 Antibody (23GB2030)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ROCK2
Long Name
Rho-associated, Coiled-coil Containing Protein Kinase 2
Alternate Names
ROCK-II, ROKalpha
Gene Symbol
ROCK2
Additional ROCK2 Products
Product Documents for ROCK2 Antibody (23GB2030)
Product Specific Notices for ROCK2 Antibody (23GB2030)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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