ROCK2 Antibody (10E1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15152
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10E1 expressed in HEK293
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human ROCK2. UniProt ID (O75116).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ROCK2 Antibody (10E1) - BSA Free (NBP3-15152) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ROCK2 Antibody (10E1) - BSA Free
![Western Blot: ROCK2 Antibody (10E1) [NBP3-15152]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-10E1-Western-Blot-NBP3-15152-img0002.jpg "Western Blot: ROCK2 Antibody (10E1) [NBP3-15152]")
Western Blot: ROCK2 Antibody (10E1) [NBP3-15152]
Western Blot: ROCK2 Antibody (10E1) [NBP3-15152] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: ROCK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 161 KDa. Observed band size: 161 KDa.![Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (10E1) [NBP3-15152]](https://resources.bio-techne.com/images/products/ROCK2-Antibody-10E1-Immunocytochemistry-Immunofluorescence-NBP3-15152-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (10E1) [NBP3-15152]")
Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (10E1) [NBP3-15152]
Immunocytochemistry/Immunofluorescence: ROCK2 Antibody (10E1) [NBP3-15152] - Immunofluorescence staining of Hela Cells with at ROCK2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for ROCK2 Antibody (10E1) - BSA Free
Application
Recommended Usage
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: ROCK2
Long Name
Rho-associated, Coiled-coil Containing Protein Kinase 2
Alternate Names
ROCK-II, ROKalpha
Gene Symbol
ROCK2
Additional ROCK2 Products
Product Documents for ROCK2 Antibody (10E1) - BSA Free
Product Specific Notices for ROCK2 Antibody (10E1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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