RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50587

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NBP3-50587-100ul
NBP3-50587-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB1180

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RNA Polymerase II/POLR2A Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-POLR2A (S5) (Uniprot: P24928)

Modification

p Ser5

Specificity

This antibody detects endogenous levels of POLR2A only when phosphorylated at S5

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)

RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)[NBP3-50587] -

Immunocytochemical staining of C2C12 cells with RNA Polymerase II/POLR2A antibody (1:1,000). Nuclei were stained blue with DAPI; RNA Polymerase II/POLR2A was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)

Flow Cytometry: RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)[NBP3-50587] -

Flow cytometric analysis of RNA Polymerase II/POLR2Aexpression in C2C12 cells using RNA Polymerase II/POLR2A antibody (1:2,000). Green, isotype control; red, Phospho-POLR2A (S5).
RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)

Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)[NBP3-50587] -

Western blotting analysis using RNA Polymerase II/POLR2A (S5) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNA Polymerase II/POLR2A

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RNA Polymerase II/POLR2A [p Ser5] Antibody (24GB1180)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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