RhoD Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04527
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 101-200 of human RhoD (NP_055393.1). YHRGQEMARSVGAVAYLEC
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit RhoD Antibody - BSA Free (NBP3-04527) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RhoD Antibody - BSA Free
![Western Blot: RhoD AntibodyAzide and BSA Free [NBP3-04527]](https://resources.bio-techne.com/images/products/RhoD Antibody-Western Blot-NBP3-04527-img0002.jpg "Western Blot: RhoD AntibodyAzide and BSA Free [NBP3-04527]")
Western Blot: RhoD AntibodyAzide and BSA Free [NBP3-04527]
Western Blot: RhoD Antibody [NBP3-04527] - Western blot analysis of extracts of various cell lines, using RhoD Rabbit pAb (NBP3-04527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: RhoD Antibody - Azide and BSA Free [RhoD] -
Immunocytochemistry/ Immunofluorescence: RhoD Antibody - Azide and BSA Free [RhoD] - Immunofluorescence analysis of MCF-7 cells using RhoD Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for RhoD Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RhoD
Alternate Names
ARHDRHOM, ras homolog D, ras homolog gene family, member A, ras homolog gene family, member D, Rho, RhoD, RhoHP1, rho-related GTP-binding protein RhoD, Rho-related protein HP1
Gene Symbol
RHOD
Additional RhoD Products
Product Documents for RhoD Antibody - BSA Free
Product Specific Notices for RhoD Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov