Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all RFC1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1148) of human Replication Factor C subunit 1 using the numbering given in entry NP_002904.3 (GeneID 5981).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RFC1 Antibody - BSA Free

Western Blot: RFC1 Antibody [NB100-230]

Western Blot: RFC1 Antibody [NB100-230]

Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: RFC1 Antibody [NB100-230]

Western Blot: RFC1 Antibody [NB100-230]

Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (50, 15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for RFC1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-6 ug/mg extract

Western Blot

1:1000-1:10000
Application Notes
This antibody can be used for Western Blot and immunoprecipitation. Suggested working dilutions: * Western Blot 1:1,000-1:10,000 Immunoprecipitation 2-6 ug/mg extract Immunocytochemistry ND Immunohistochemistry ND *The investigator should determine the optimal dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RFC1

The protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability. [provided by RefSeq]

Long Name

Replication Factor C 1

Alternate Names

MHCBFB, PO-GA, RECC1, RFC140

Entrez Gene IDs

5981 (Human)

Gene Symbol

RFC1

UniProt

NP_002904.3 (Human)

Additional RFC1 Products

Product Documents for RFC1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RFC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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