RBM25 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83418

Novus Biologicals, part of Bio-Techne
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NBP1-83418
NBP1-83418-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RBM25 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PVDSVFNKFEDEDSDDVPRKRKLVPLDYGEDDKNATKGTVNTEEKRKHIKSLIEKIPTAKPELFAYPLDWSIVDSILMERRIRPWINKKIIEYIGEEEATLVDFVCSKVMAHSSPQSILDDVAMVLDEEAEV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit RBM25 Antibody - BSA Free (NBP1-83418) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for RBM25 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RBM25 Antibody [NBP1-83418]

Immunocytochemistry/ Immunofluorescence: RBM25 Antibody [NBP1-83418]

Immunocytochemistry/Immunofluorescence: RBM25 Antibody [NBP1-83418] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: RBM25 Antibody [NBP1-83418]

Immunohistochemistry-Paraffin: RBM25 Antibody [NBP1-83418]

Immunohistochemistry-Paraffin: RBM25 Antibody [NBP1-83418] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: RBM25 Antibody [NBP1-83418]

Immunohistochemistry-Paraffin: RBM25 Antibody [NBP1-83418]

Immunohistochemistry-Paraffin: RBM25 Antibody [NBP1-83418] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
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Applications for RBM25 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:25

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in MOLT-4 and A431 lysates, separated by Size, antibody dilution of 1:25, apparent MW was 141-144 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBM25

RBM25, also identified as hRED120 (for human Arg/Glu/Asp-rich protein of 120 kDa), contains an RNA recognition motif and a PWI nucleic acid binding domain and is suggested to have a role in RNA processing.

Alternate Names

Arg/Glu/Asp-rich protein of 120 kDa, Arg/Glu/Asp-rich protein, 120 kDa, fSAP94, functional spliceosome-associated protein 94, MGC105088, MGC117168, NET52, Protein S164, RED120RNA-binding region-containing protein 7, RNA binding motif protein 25, RNA-binding motif protein 25, RNA-binding protein 25, RNA-binding region (RNP1, RRM) containing 7, RNPC7, S164, Snu71, U1 small nuclear ribonucleoprotein 1SNRP homolog

Gene Symbol

RBM25

Additional RBM25 Products

Product Documents for RBM25 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RBM25 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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