Vanilloid R1/TRPV1 was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Goat Anti-Rat Vanilloid R1/TRPV1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3066) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Vanilloid R1/TRPV1 in Rat Brain.

Vanilloid R1/TRPV1 was detected in perfusion fixed frozen sections of rat brain using Rat Vanilloid R1/TRPV1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3066) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Rat Vanilloid R1/TRPV1 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat dorsal root ganglion and rat brain

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vanilloid R1/TRPV1

VR1, also known as TRPV1 (Transient Receptor Potential Vanilloid 1) and capsaicin receptor, is a 115 kDa integral membrane ion channel that has six transmembrane domains and contains intracellular N-and C-termini. It contributes to normal pain and temperature sensation and also has a “sensory-effector” function. By alternative splicing, at least three VR1 isoforms are known. The sequence of residues 4-21 of rat VR1 is 78% and 28% identical to that of mouse and human VR1, respectively.

Long Name

Vanilloid Receptor 1/Transient Receptor Potential Cation Channel 1

Alternate Names

Capsaicin Receptor, TRPV1, VanilloidR1, VR1

Entrez Gene IDs

7442 (Human); 193034 (Mouse); 83810 (Rat)

Gene Symbol

TRPV1

UniProt

O35433

Additional Vanilloid R1/TRPV1 Products

Product Documents for Rat Vanilloid R1/TRPV1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat Vanilloid R1/TRPV1 Antibody

For research use only

Citations
Reviews

Rat Vanilloid R1/TRPV1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3066

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Vanilloid R1/TRPV1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat Vanilloid R1/TRPV1 Antibody

Vanilloid R1/TRPV1 in Rat Dorsal Root Ganglion.