Rat Neuropilin-1 Antibody

Name: Rat Neuropilin-1 Antibody
Brand: R&D Systems
SKU: MAB566
Price: 160 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB566

Citations (2)

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Key Product Details

Species Reactivity:	Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1 Phe22-Asp854 (Lys811Arg, Pro812-Gly828 del), predicted Accession # Q9QWJ9
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 130603
Specificity:	Detects rat Neuropilin-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat Neuropilin-2 is observed.

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Applications for Rat Neuropilin-1 Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Rat cortical cells
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of Recombinant Human VEGF₁₆₅ (Catalog # 293-VE) to immobilized Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) coated at 2 µg/mL (100 µL/well). At 5 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Neuropilin-1

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins.

Item	Value
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Product Specific Notices for Rat Neuropilin-1 Antibody

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Rat Neuropilin-1 Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Rat Neuropilin-1 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Wanami LS et al. (2008), Vascular endothelial growth factor-A stimulates Snail expression in breast tumor cells: implications for tumor progression. Exp. Cell Res., 314(13):2448-53.
PMID: 18554584
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry

Hacquard-Bouder C et al. (2007), Alteration of antigen-independent immunologic synapse formation between dendritic cells from HLA-B27-transgenic rats and CD4+ T cells: selective impairment of costimulatory molecule engagement by mature HLA-B27. Arthritis Rheum., 56(5):1478-89.
PMID: 17469106

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