Rat IL-1Rrp2/IL-1R6 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF573S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Western Blot
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat IL‑1 Rrp2/IL‑1 R6
Gly22-Tyr340
Accession # Q62929
Gly22-Tyr340
Accession # Q62929
Specificity
Detects rat IL‑1 Rrp2/IL‑1 R6 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant human (rh) IL‑1 R6 is observed and less than 5% cross‑reactivity with rhIL-18 R beta, rhIL-1 RI, a
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Rat IL-1Rrp2/IL-1R6 Alexa Fluor® 750-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: IL-1Rrp2/IL-1R6
The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL‑18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll‑like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Rat IL-1 Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 319 aa extracellular domain. It shares 84% and 67% amino acid sequence identity with mouse and human IL‑1 Rrp2, respectively. IL-1 Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL‑1 Rrp2 has been shown to mediate the activation of the transcription factor NF‑kB by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1e), with IL‑1 RAcP as a co-factor (3, 4). Response to IL-1 F9 is specifically antagonized by IL-1 F5 (also known as IL-1d), an IL-1 family ligand that is most closely related to IL‑1ra (3). IL-1 Rrp2, IL-1 F5, and IL-1 F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1 Rrp2 mediated signaling pathway may take part in local inflammatory responses (3).
Long Name
Interleukin 1 Receptor-like 2
Alternate Names
IL-1 R6, IL-1 Rrp2, IL-1RL2, IL-1Rrp2, IL1RL2, IL1Rrp2
Gene Symbol
IL1RL2
UniProt
Additional IL-1Rrp2/IL-1R6 Products
Product Specific Notices for Rat IL-1Rrp2/IL-1R6 Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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