Western blot shows lysates of rat adipose tissue and rat mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Rat Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB3100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Adiponectin/Acrp30 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Adiponectin/Acrp30 in Rat Adipocytes.

Adiponectin/Acrp30 was detected in Immersion fixed rat mesenchymal stem cells differentiated into adipocytes with Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011) using Mouse Anti-Rat Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Rat Adiponectin/Acrp30 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011)

Western Blot

1 µg/mL
Sample: Rat adipose tissue and rat mammary gland tissue

Reviewed Applications

Read 1 review rated 5 using MAB3100 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponectin/Acrp30

Adiponectin, also known as Adipocyte complement-related protein of 30 kDa (Acrp30), is a secreted adipose tissue-specific protein. It is an anti-atherogenic, anti-diabetic and anti-inflammatory hormone that plays important roles in the regulation of lipid and glucose metabolism. Rat Adiponectin/Acrp30 shares 91% and 83% amino acid sequence homology with mouse and human Adiponectin/Acrp30, respectively.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Gene Symbol

ADIPOQ

UniProt

NP_653345

Additional Adiponectin/Acrp30 Products

Product Documents for Rat Adiponectin/Acrp30 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Rat Adiponectin/Acrp30 Antibody

For research use only

Citations
Reviews

Rat Adiponectin/Acrp30 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3100

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Adiponectin/Acrp30 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat Adiponectin/Acrp30 Antibody

Detection of Rat Adiponectin/Acrp30 by Western Blot.