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Rad51C Antibody (2H11/6) - BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-177

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Primate, Yeast

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2H11/6

Format

BSA Free

Concentration

1 mg/ml

Product Summary for Rad51C Antibody (2H11/6) - BSA Free

Immunogen

His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502]

Localization

Nuclear

Specificity

Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad51C Antibody (2H11/6) - BSA Free

Western Blot: Rad51C Antibody (2H11/6) - BSA Free [NB100-177] - The expression of DNA damage-responsive proteins was measured by western blot analysis following treatment with olaparib and SAHA alone or in combination. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25888415/) licensed under a CC-BY license.
Flow Cytometry: Rad51C Antibody (2H11/6) [NB100-177] - Intracellular flow cytometric staining of 1 x 10^6 HeLa cells using Rad51C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Rad51C detected in HEK293 lysate using NB 100-177. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School.

Applications for Rad51C Antibody (2H11/6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 23512992)

Simple Western

1:100

Western Blot

1:1000
Application Notes
In WB, a band can be seen at approx. 40 kDa. Preliminary feedback has been negative for Immunofluorescence on 4% PFA-fixed human cell lines (H1299 and MCF7).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 35 publications using NB100-177 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rad51C

The Rad51 DNA repair protein is a key component of the double-strand break repair pathway and is essential for mitotic and meiotic recombination and genomic stability. Five human Rad51 homologs have been identified: XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex, or with multiple smaller complexes.

Alternate Names

BROVCA3, DNA repair protein RAD51 homolog 3, FANCO, MGC104277, R51H3, RAD51 homolog C, RAD51 homolog C (S. cerevisiae), RAD51L2RAD51 (S. cerevisiae) homolog C, RAD51-like protein 2, yeast RAD51 homolog 3

Gene Symbol

RAD51C

Additional Rad51C Products

Product Documents for Rad51C Antibody (2H11/6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rad51C Antibody (2H11/6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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