Purine Nucleoside Phosphorylase/PNP Antibody (103) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90053
Recombinant Monoclonal Antibody
![Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053]](https://resources.bio-techne.com/images/products/Purine-Nucleoside-Phosphorylase-PNP-Antibody-103-Western-Blot-NBP2-90053-img0002.jpg "Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 103 expressed in HEK293
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Purine Nucleoside Phosphorylase/PNP (Uniprot#: P00491; Met 1-Ser 289).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images
Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053]
Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa![Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053]](https://resources.bio-techne.com/images/products/Purine-Nucleoside-Phosphorylase-PNP-Antibody-103-Immunoprecipitation-NBP2-90053-img0001.jpg "Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053]")
Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053]
Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-PNP-3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PNP-3 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 33 kDaApplications
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Purine Nucleoside Phosphorylase/PNP
Alternate Names
Inosine phosphorylase, PUNP
Gene Symbol
PNP
Additional Purine Nucleoside Phosphorylase/PNP Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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