PTIP Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-577
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human PAX transcription activation domain interacting protein 1 using the numbering given in Jowsey et akl., 2004, J. Biol. Chem. 279(53):55562-55569 (GeneID 22976).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PTIP Antibody - BSA Free (NB100-577) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PTIP Antibody - BSA Free
Western Blot: PTIP Antibody [NB100-577]
Western Blot: PTIP Antibody [NB100-577] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PTIP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 secondsImmunoprecipitation: PTIP Antibody [NB100-577]
Immunoprecipitation: PTIP Antibody [NB100-577] - Detection of human PTIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTIP antibody NB100-577 used for IP at 3 ug per reaction. PTIP was also immunoprecipitated by rabbit anti-PTIP antibody from Company B. For blotting immunoprecipitated PTIP, NB100-577 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for PTIP Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PTIP
Alternate Names
CAGF28, PACIP1, PAX interacting (with transcription-activation domain) protein 1, PAX transactivation activation domain-interacting protein, PAX transcription activation domain interacting protein 1 like, PAX-interacting protein 1, PAXIP1LCAGF29, protein encoded by CAG trinucleotide repeats, PTIPFLJ41049, TNRC2
Entrez Gene IDs
22976 (Human)
Gene Symbol
PAXIP1
Additional PTIP Products
Product Documents for PTIP Antibody - BSA Free
Product Specific Notices for PTIP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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