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Key Product Details

Species Reactivity

Porcine

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 377537

Product Summary for Porcine CD31/PECAM‑1 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant porcine CD31/PECAM‑1
Gln28-Lys602 (predicted)
Accession # Q95242

Specificity

Detects porcine CD31/PECAM‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CD31, recombinant mouse (rm) CD31, rhVCAM-1, rhICAM-1, rhICAM-2, rhICAM-3, rmICAM-5, or rmMADCAM-1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Porcine CD31/PECAM‑1 Antibody

Detection of CD31/PECAM-1 antibody in Porcine PBMCs antibody by Flow Cytometry.

Detection of CD31/PECAM‑1 in Porcine PBMCs by Flow Cytometry.

Porcine peripheral blood mononuclear cells were stained with Rat Anti-Porcine CD31/PECAM-1 Monoclonal Antibody (Catalog # MAB33871, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).

Applications for Porcine CD31/PECAM‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Porcine peripheral blood mononuclear cells

Western Blot

1 µg/mL
Sample: Recombinant Porcine CD31/PECAM‑1 (Catalog # 3387-PC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB33871 in the following applications:

Published Applications

Read 4 publications using MAB33871 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD31/PECAM-1

CD31, also known as PECAM-1 (platelet-endothelial cell adhesion molecule-1), is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to the vascular system, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. CD31 is concentrated at cell-cell junctions and is required for transendothelial migration (TEM) (1‑3). The extracellular domain (ECD) of CD31 has ten potential N-glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains immunoregulatory tyrosine-based inhibitory and switch motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum CD31 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue‑ and stage-specific, but full-length CD31 is predominant. A form lacking the ITSM predominates in mouse (9). Porcine CD31 ECD shows 74%, 73%, 70%, 63% and 62% amino acid (aa) identity with bovine, canine, human, mouse and rat CD31, respectively. CD31 participates with other adhesion molecules for most functions but is the critical molecule for TEM. Homotypic CD31 adhesion in trans combined with cycling of CD31 to and from surface-connected endothelial cell vesicles leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. CD31-/- mice are deficient in chemokine-mediated chemotaxis (14).

References

  1. Ilan, N. and J.A. Madri (2003) Curr Opin. Cell Biol. 15:515.
  2. Nasu, K. et al. (1999) Transplantation 68:861.
  3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
  4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
  5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
  6. Ilan, N. et al. (2001) FASEB J. 15:362.
  7. Eugenin, E.A. et al. (2006)J. Leukoc. Biol. 79:444.
  8. Losy, J. et al. (1999) 99:169.
  9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
  10. Mamdouh, Z. et al. (2003) Nature 421:748.
  11. Gao, C. et al. (2003) Blood 102:169.
  12. Falati, S. et al. (2006) Blood 107:535.
  13. Wee, J. L. and D.E. Jackson (2005) Blood 106:3816.
  14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Gene Symbol

PECAM1

UniProt

Product Documents for Porcine CD31/PECAM‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Porcine CD31/PECAM‑1 Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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