PKC eta Antibody (24GB1480)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50603

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NBP3-50603-100ul
NBP3-50603-20ul
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB1480

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKC eta Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PKC eta (Uniprot: P24723)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC eta Antibody (24GB1480)

PKC eta Antibody (24GB1480)

Immunocytochemistry/ Immunofluorescence: PKC eta Antibody (24GB1480)[NBP3-50603] -

Immunocytochemical staining of HepG2 cells with PKC eta antibody (1:1,000). Nuclei were stained blue with DAPI; PKC eta was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
PKC eta Antibody (24GB1480)

Flow Cytometry: PKC eta Antibody (24GB1480)[NBP3-50603] -

Flow cytometric analysis of PKC etaexpression in HepG2 cells using PKC eta antibody (1:2,000). Green, isotype control; red, Protein kinase C eta.
PKC eta Antibody (24GB1480)

Western Blot: PKC eta Antibody (24GB1480)[NBP3-50603] -

Western blotting analysis using PKC eta antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for PKC eta Antibody (24GB1480)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKC eta

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

Long Name

Protein kinase C eta type

Alternate Names

EC 2.7.11.13, nPKC-eta, PKC-L, PKCL, PRKCH

Gene Symbol

PRKCH

Additional PKC eta Products

Product Documents for PKC eta Antibody (24GB1480)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC eta Antibody (24GB1480)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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