Peroxidase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50129

Novus Biologicals, part of Bio-Techne
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NBP3-50129
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Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Peroxidase Antibodies »

Product Specifications

Immunogen

Peroxidase (Horseradish)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against stated buffer.

Applications for Peroxidase Antibody

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody has been tested by ELISA and western blot and is suitable for dot blot, immunoprecipitation, and most immunological methods requiring high titer and specificity.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Peroxidase

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric.

Alternate Names

EC 1.11.1.7, Horseradish Peroxidase, HPRC 1, HPRC1, HRPA2, HRPC 2, HRPC2, HRPE5, HRPN, Neutral peroxidase, Peroxidase A2, Peroxidase C1A, Peroxidase C1B, Peroxidase C2, Peroxidase C3, Peroxidase E5, Peroxidase N, PRXC1A, PRXC1B, PRXC2, PRXC3

Additional Peroxidase Products

Product Documents for Peroxidase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Peroxidase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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