PCCB Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85886

Novus Biologicals, part of Bio-Techne
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NBP1-85886
NBP1-85886-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCCB Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKFANPFPAAVRGFVDDIIQPSSTRARICCDLDVL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PCCB Antibody - BSA Free (NBP1-85886) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-PCCB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PCCB Antibody - BSA Free

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining in human liver and skin tissues . Corresponding PCCB RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human kidney, liver, salivary gland and tonsil using Anti-PCCB antibody NBP1-85886 (A) shows similar protein distribution across tissues to independent antibody NBP1-85887 (B).
Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human kidney using Anti-PCCB antibody NBP1-85886.
View 11 more images

Applications for PCCB Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 58 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCCB

PCCB is encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). [provided by RefSeq]

Alternate Names

beta polypeptide, mitochondrial, propionyl CoA carboxylase, beta polypeptide

Entrez Gene IDs

5096 (Human)

Gene Symbol

PCCB

UniProt

P05166 (Human)

Additional PCCB Products

Product Documents for PCCB Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PCCB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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