PAK1/2/3 [p Ser154] Antibody (24GB3960)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50762

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NBP3-50762-100ul
NBP3-50762-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB3960

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAK1/2/3 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-PAK1/2/3 (S144+S141+S154) (Uniprot: Q13153/Q13177/O75914)

Modification

p Ser154

Specificity

This antibody detects endogenous levels of PAK1/PAK2/PAK3 only when phosphorylated at S144 and S141 and S154

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK1/2/3 [p Ser154] Antibody (24GB3960)

PAK1/2/3 [p Ser154] Antibody (24GB3960)

Immunocytochemistry/ Immunofluorescence: PAK1/2/3 [p Ser154] Antibody (24GB3960)[NBP3-50762] -

Immunocytochemical staining of H9C2 cells with PAK1/2/3 antibody(1:1,000). Nuclei were stained blue with DAPI; PAK1/2/3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 um.
PAK1/2/3 [p Ser154] Antibody (24GB3960)

Flow Cytometry: PAK1/2/3 [p Ser154] Antibody (24GB3960)[NBP3-50762] -

Flow cytometric analysis of PAK1/2/3expression in H9c2 cells using PAK1/2/3 antibody (1:2,000). Green, isotype control; red, Phospho-PAK1/2/3 (S144+S141+S154).
PAK1/2/3 [p Ser154] Antibody (24GB3960)

Western Blot: PAK1/2/3 [p Ser154] Antibody (24GB3960)[NBP3-50762] -

Western blotting analysis using PAK1/2/3 (S144+S141+S154) antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for PAK1/2/3 [p Ser154] Antibody (24GB3960)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAK1/2/3

In mammals, there are several identified isoforms of p21-activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously. Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death. Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK. (1-4)

Alternate Names

Alpha-PAK, EC 2.7.11, EC 2.7.11.1, MGC130000, MGC130001, p21 protein (Cdc42/Rac)-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related), p21-activated kinase 1, p65-PAK, PAK-1, PAKalpha, serine/threonine-protein kinase PAK 1, STE20 homolog, yeast

Gene Symbol

PAK1

Additional PAK1/2/3 Products

Product Documents for PAK1/2/3 [p Ser154] Antibody (24GB3960)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PAK1/2/3 [p Ser154] Antibody (24GB3960)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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