Oxidized PTP Active Site Antibody (335636) [mFluor Violet 500 SE]
Novus Biologicals, part of Bio-Techne | Catalog # FAB2844MFV500
Conjugate
Catalog #
Key Product Details
Species Reactivity
Multi-Species
Applications
Western Blot, Neutralization, Immunoprecipitation
Label
mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 335636
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
KLH-coupled perfomic acid oxidized synthetic peptide
VHCSAG
VHCSAG
Specificity
Detects Oxidized PTP Active Site. Will react with any oxidized phosphatase containing the consensus sequence. Detects oxidized but not unoxidized DEP-1, PTP1B, TC-PTP, and SHP-2. Other phosphatases have not been tested.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Oxidized PTP Active Site Antibody (335636) [mFluor Violet 500 SE]
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Oxidized PTP Active Site
Oxidation of the essential cysteine in the active site of protein tyrosine phosphatases (PTPs) is believed to play an important role in regulating the activity of these enzymes. Rapid, oxidant-dependent inhibition of phosphatases such as PTP1B, TC-PTP and SHP-2 may play a role in fine tuning the responsiveness of receptor tyrosine kinases for growth factors. The initial oxidation to a sulfenic acid (-SOH) is reversible, but oxidation can progress to an irreversible sulfonic acid (-SO3H) that can be detected by antibodies made against an oxidized peptide containing the PTP active site consensus sequence.
Long Name
Oxidized Protein Tyrosine Phosphatase Active Site
Additional Oxidized PTP Active Site Products
Product Documents for Oxidized PTP Active Site Antibody (335636) [mFluor Violet 500 SE]
Product Specific Notices for Oxidized PTP Active Site Antibody (335636) [mFluor Violet 500 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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