Oxidized PTP Active Site Antibody (335636) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB2844CL1
![Oxidized PTP Active Site Antibody (335636) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/fab2844cl1_mouse-mab-335636-corafluor-1-26920251750610.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Multi-Species
Applications
Western Blot, Neutralization, Immunoprecipitation
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # 335636
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
KLH-coupled perfomic acid oxidized synthetic peptide
VHCSAG
VHCSAG
Specificity
Detects Oxidized PTP Active Site. Will react with any oxidized phosphatase containing the consensus sequence. Detects oxidized but not unoxidized DEP-1, PTP1B, TC-PTP, and SHP-2. Other phosphatases have not been tested.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for Oxidized PTP Active Site Antibody (335636) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Oxidized PTP Active Site Antibody (335636) [CoraFluor™ 1]
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Oxidized PTP Active Site
Oxidation of the essential cysteine in the active site of protein tyrosine phosphatases (PTPs) is believed to play an important role in regulating the activity of these enzymes. Rapid, oxidant-dependent inhibition of phosphatases such as PTP1B, TC-PTP and SHP-2 may play a role in fine tuning the responsiveness of receptor tyrosine kinases for growth factors. The initial oxidation to a sulfenic acid (-SOH) is reversible, but oxidation can progress to an irreversible sulfonic acid (-SO3H) that can be detected by antibodies made against an oxidized peptide containing the PTP active site consensus sequence.
Long Name
Oxidized Protein Tyrosine Phosphatase Active Site
Additional Oxidized PTP Active Site Products
Product Documents for Oxidized PTP Active Site Antibody (335636) [CoraFluor™ 1]
Product Specific Notices for Oxidized PTP Active Site Antibody (335636) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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